Services

Fully post-translational modified mammalian recombinant antibodies (rAb’s) are produced via the rAb expression platform. Antibody variable domains can be seamlessly ligated in antibody expression vectors to generate each antibody class.

Gram amounts of purified antibodies with low levels of endotoxin (< 0.05 EU/mg) can be provided in 4 to 6 weeks. The recombinant antibody production comprises the following steps:

The rAb platform ahas a modular architecture, thereby allowing several transfection and delivery options. For more information on the transfection and delivery options, please send us an email.

Rapid Prime is ImmunoPrecise’s proprietary custom monoclonal antibody development service. This rapid immunization strategy saves weeks in development time. When coupled with the Single-Step cloning procedure, positive hybridoma cell lines are identified in 65 days. This rapid immunization procedure does not compromise on affinity. It consistently generates large numbers of IgG clones specific for antigen.

IPA’s proprietary Rapid Prime Method in mice identifies positive monoclonal antibodies in as little as 32 days. While this method also uses Single-Step cloning to accelerate the process, it is especially good for generating anti-idiotypic antibodies and has also been extremely useful for generating monoclonal antibodies against conformational epitopes. Other sources of immunogens include conserved or smaller proteins, peptides, and whole cell bacteria.

More information about the Rapid Prime method can be found here.

ImmunoPrecise’s Standard Method monoclonal antibody development service in mice and rats is fast and efficient. IPA’s single-step cloning method shaves weeks off of the traditional time line for hybridoma production. IPA custom develops monoclonal antibodies to an array of antigens including peptides, modified peptides, recombinant proteins, DNA and small molecules.

More information about the Standard Method monoclonal antibody development can be found here.

ImmunoPrecise Antibodies offers custom Rabbit Monoclonal Antibody development service using RMAT (Recombinant Monoclonal Antibody Technology). This proprietary technology screens the repertoire of the rabbit creating the power to select the desired antibody directly from B cells.

The RMAT Advantage

• High affinity antibodies without the traditional cell fusion step; avoids stability issues commonly associated with hybridomas
• Recombinant antibody ensures preservation of genetic information
• Both a monoclonal and polyclonal end product
• Utilization of rabbit immune system to generate antibodies against different epitopes on the same antigen

More information about the Rabbit monoclonal antibody development can be found here.

ImmunoPrecise is a leading producer of high-titre custom mouse, rat, rabbit, goat, and sheep polyclonal antibodies. We offer a comprehensive range of services to get you the polyclonal product you need:

• Epitope prediction
• Custom peptide synthesis
• Conjugation
• Immunization
• Purification
• Assay design

More information about the Polyclonal development can be found here.

The technology behind the products and services provided by QVQ is based on the smallest naturally occurring antibody-based molecules, called VHH or Nanobdy (~15 kDa). Next to the small size, VHH are highly soluble, show the ability to refold to their functional structure after denaturation and are able to target alternative epitopes, which are not targeted by conventional monoclonal antibodies and their minimized versions. VHHs can be obtained from the immunization of llamas.