U-Protein Express BV has collaborated with or provided recombinant protein to several scientific organizations resulting in the following publications.


Mutational signature in colorectal cancer caused by genotoxic pks+ E. coli, C. Pleguezuelos-Manzano, J. Puschhof, A.R. Huber, A. van Hoeck, H.M. Wood, J. Nomburg, C. Gurjao, F. Manders, G. Dalmasso, P.B. Stege, F.L. Paganelli, M.H. Geurts, J. Beumer, T. Mizutani, R. van der Linden, S. van Elst, Genomics England Research Consortium, J. Top, R.J.L. Willems, M. Giannakis, R. Bonnet, P. Quirke, M. Meyerson, E. Cuppen, R. van Boxtel, and H. Clevers, Nature, 2020.

CRISPR-Based Adenine Editors Correct Nonsense Mutations in a Cystic Fibrosis Organoid Biobank, M.H.Geurts, E. Poel, G.D. Amatngalim, R. Oka, F.M. Meijers, E. Kruisselbrink, P. van Mourik, G. Berkers, K.M. de Winter-de Groot, S. Miche, D. Muilwijk, B.L. Aalbers, J. Mullenders, S.F. Boj, S.W.F. Suen, J.E. Brunsveld, H.M. Janssens, M.A. Mall, S.Y. Graeber, R. van Boxtel, C.K. van der Ent, J.M. Beekman, and Hans Clevers, Cell Stem Cell, 2020.

Cysteamine-bicalutamide combination treatment restores alpha-ketoglutarate and corrects proximal tubule phenotype in cystinosis, A. Jamalpoor, C.A.G.H. van Gelder, F.A. Yousef Yengej, E.A. Zaal, S. Princiero Berlingerio, K.R. Veys, C.P. Casellas, K. Voskuil, K. Essa, C.M.E. Ammerlaan, L.R. Rega, R. van der Welle, M.R. Lilien, M.B. Rookmaaker, H. Clevers, J. Klumperman, E. Levtchenko, C.R. Berkers, M.C. Verhaar, M. Altelaar, R. Masereeuw, and M.J. Janssen, BioRxiv, 2020.

Outer membrane permeabilization by the membrane attack complex sensitizes Gram negative bacteria to antimicrobial proteins in serum and phagocytes, D.A.C. Heesterbeek, R.M. Muts, V.P. van Hensbergen, P. de Saint Aulaire, T. Wennekes, B.W. Bardoel, N.M. van Sorge and S.H.M. Rooijakkers, BioRxiv, 2020.

Whole blood thrombin generation profiles of patients with cirrhosis explored with a near patient assay, J. Wan, L.N. Roberts, W. Hendrix, J. Konings, T.W. Ow, L. Rabinowich, O. Barbouti, B. de Laat, R. Arya, V.C. Patel, M. Roest, T. Lisman, and W. Bernal, Journal of Thrombosis and Haemostasis, 2020.

Snake Venom Gland Organoids, Y. Post, H. Clevers, et al., Cell, 2020.


Novel Aptamer To Von Willebrand Factor A1 Domain (TAGX-0004) Shows Total Inhibition Of Thrombus Formation Superior To ARC1779 And Comparable To Caplacizumab, K. Sakai, T. Someya, K. Harada, H. Yagi, T. Matsui, M. Matsumoto, Haematologica, 2019.

Role of GnRH isoforms in paracrine/autocrine control of zebrafish (Danio rerio) spermatogenesis, H.P. Fallah, M.S. Rodrigues, S. Corchuelo, R.H. Nóbrega, and H.R. Habibi, Endocrinology, 2019.

Design and structural characterisation of olfactomedin-1 variants as tools for functional studies, M.F. Pronker, H. van den Hoek, and, B. Janssen, BMC Molecular and Cell Biology, 2019.

Patient-Derived Head and Neck Cancer Organoids Recapitulate EGFR Expression Levels of Respective Tissues and Are Responsive to EGFR-Targeted Photodynamic Therapy, E. Driehuis, S. Spelier, I. Beltrán Hernández, R. de Bree, S.M. Willems, H. Clevers, and, S. Oliveira, Journal of Clinical Medicine, 2019.

Novel Assays to Distinguish Between Properdin-Dependent and Properdin-Independent C3 Nephritic Factors Provide Insight Into Properdin-Inhibiting Therapy, M.A.H.M. Michels, N.C.A.J. van de Kar, R.M. van den Bos, T.J.A.M. van der Velden, S.A.W. van Kraaij, S.A. Sarlea, V. Gracchi, M.J.S. Oosterveld, E.B. Volokhina, and L.P.W.J. van den Heuvel, Frondiers in Immunology, 2019.

Enhanced Antiviral Activity of Human Surfactant Protein D by Site-Specific Engineering of the Carbohydrate Recognition Domain, M. van Eijk, M. Hillaire, G. Rimmelzwaan, M. Rynkiewicz, M.R. White, K. Hartshorn, M. Hessing, P. Koolmees, M. Tersteeg, M. van Es, T. Meijerhof, A. Huckriede, and Henk P. Haagsman, Frondiers in Immunology, 2019.

Insights Into Enhanced Complement Activation by Structures of Properdin and Its Complex With the C-Terminal Domain of C3b, R.M. van den Bos, N.M. Pearce, J. Granneman, T.H.C. Brondijk, and P. Gros, Frondiers in Immunology, 2019.

Development of a Stable Respiratory Syncytial Virus Pre-Fusion Protein Powder Suitable for a Core-Shell Implant with a Delayed Release in Mice: A Proof of Concept Study, M. Beugeling, K. Amssoms, F. Cox, B. De Clerck, E. Van Gulck, J.A. Verwoerd, G. Kraus, D. Roymans, L. Baert, H.W. Frijlink, and W.L.J. Hinrichs, pharmaceutics, 2019.

Oral Mucosal Organoids as a Potential Platform for Personalized Cancer Therapy, E. Driehuis, S. Kolders, S. Spelier, K. Lõhmussaar, S.M. Willems, L.A. Devriese, R. de Bree, E.J. de Ruiter, J. Korving, H. Begthel, J.H. van Es, V. Geurts, G.W. He, R.H. van Jaarsveld, R. Oka, M.J. Muraro, J. Vivié, M.M.J.M. Zandvliet, A.P.A. Hendrickx, N. Iakobachvili, P. Sridevi, O. Kranenburg, R. van Boxtel, G.J.P.L. Kops, D.A. Tuveson, P.J. Peters, A. van Oudenaarden, and Hans Clevers, Cancer Discovery, 2019.

Identification of Enteroendocrine Regulators by Real-Time Single-Cell Differentiation Mapping, H.Gehart, J.H. van Es, K. Hamer, J. Beumer, K. Kretzschmar, J.F. Dekkers, Anne Rios, and H. Clevers, Cell, 2019.

Met inhibition revokes IFNγ-induction of PD-1 ligands in MET-amplified tumours, V. Martin, C. Chiriaco, C. Modica, A. Acquadro, M. Cortese, F. Galimi, T. Perera, L. Gammaitoni, M. Aglietta, P.M. Comoglio, E. Vigna, and D. Sangiolo, British Journal of Cancer, 2019.

Tubuloids derived from human adult kidney and urine for personalized disease modeling, F. Schutgens, M.B. Rookmaaker, T. Margaritis, A. Rios, C. Ammerlaan, J. Jansen, L. Gijzen, M. Vormann, A. Vonk, M. Viveen, F.Y. Yengej, S. Derakhshan, K.M. de Winter-de Groot, B. Artegiani, R. van Boxtel, E. Cuppen, A.P.A. Hendrickx, M.M. van den Heuvel-Eibrink, E. Heitzer, H. Lanz, J. Beekman, J.L. Murk, R. Masereeuw, F. Holstege, J. Drost, M.C. Verhaar, and H. Clevers, Nature Biotechnology, 2019.

Affibody-Mediated Sequestration of Amyloid β Demonstrates Preventive Efficacy in a Transgenic Alzheimer’s Disease Mouse Model, A. Boutajangout, H. Lindberg, A. Awwad, A. Paul1, R. Baitalmal, I. Almokyad, I. Höidén-Guthenberg, E. Gunneriusson, F.Y. Frejd, T. Härd, J. Löfblom, S. Ståhl and T. Wisniewski, Frontiers Aging Neuroscience, 2019.

A novel L1CAM isoform with angiogenic activity generated by NOVA2-mediated alternative splicing, F. Angiolini et al., eLife, 2019.

Complement-dependent outer membrane perturbation sensitizes Gram-negative bacteria to Gram-positive specific antibiotics, D.A.C. Heesterbeek, N.I. Martin, A. Velthuizen, M. Duijst, M. Ruyken, R. Wubbolts, S.H.M. Rooijakkers, and B.W. Bardoel, Scientific Reports, 2019.

Four individually druggable MET hotspots mediate HGF-driven tumor progression, C. Basilico, A. Hultberg, C. Blanchetot, N. de Jonge, E. Festjens, V. Hanssens, S. Osepa, G. De Boeck, A. Mira, M. Cazzanti, V. Morello, T. Dreier, M. Saunders, H. de Haard, and P. Michieli, The Journal of Clinical Investigation, 2019.

Met inhibition revokes IFNγ-induction of PD-1 ligands in MET-amplified tumours, V. Martin, C. Chiriaco, C. Modica, A. Acquadro, M. Cortese, F. Galimi, T. Perera, L. Gammaitoni, M. Aglietta, P.M. Comoglio, E. Vigna, and D. Sangiolo, British journal of Cancer, 2019.

Analytical characterization and reference interval of an enzyme-linked immunosorbent assay for active von Willebrand factor, L.N. van der Vorm, L. Li, D. Huskens, W. Chayoua, H. Kelchtermans, P.G. de Groot, M. Roest, J.A. Remijn, B. de Laat, PLOS ONE, 2019.

Bacterial killing by complement requires membrane attack complex formation via surface-bound C5 convertases, D.A.C. Heesterbeek, B.W. Bardoel, E.S. Parsons, I. Bennett, M. Ruyken, D.J. Doorduijn, R.D. Gorham Jr, E.T.M. Berends, A.L.B. Pyne, B.W. Hoogenboom, and S.H.M. Rooijakkers, The EMBO Journal, 2019.

Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells, N. Fenderico, R.C. van Scherpenzeel, M. Goldflam, D. Proverbio, I. Jordens, T. Kralj, S. Stryeck, T.Z. Bass, G. Hermans, C. Ullman, T. Aastrup, P. Gros, and M.M. Maurice, Nature Communications, 2019.

Structure and flexibility of the extracellular region of the PirB receptor, H.C. Vlieg, E.G. Huizinga, and B.J.C. Janssen, JBC, 2019.


Mix and match of the tumor metastasis suppressor Nm23protein isoformsin vitroandin vivo, C.M. Potel, D. Fasci, and A.J.R. Heck, FEBS, 2018.

IL4-10 fusion protein has chondroprotective, anti-inflammatory and potentially analgesic effects in the treatment of osteoarthritis, C. Steen-Louws, J. Popov-Celeketic, S.C.Mastbergen, K. Coeleveld, C.E. Hack, N. Eijkelkamp, M. Tryfonidou, S. Spruijt, J.A.G.van Roon, and F.P.J.G. Lafeber, Osteoarthritis and Cartilage, 2018.

Mass Spectrometry in the Determination of Glycosylation Site and N-Glycan Structures of Human Placental Alkaline Phosphatase, K. Solakyildirim, L. Li, and R.J. Linhardt, Mass Spectrometry Letters, 2018.

Unbiased Combinatorial Screening Identifies a Bispecific IgG1 that Potently Inhibits HER3 Signaling via HER2-Guided Ligand Blockade, C.A.W.Geuijen, C. De Nardis, D. Maussang, E. Rovers, T. Gallenne, L.J.A. Hendriks, T. Visser, R. Nijhuis, T. Logtenberg, J. Kruif, P. Gros, and M. Throsby, Cancer Cell, 2018.

Structural basis of latent TGF-β1 presentation and activation by GARP on human regulatory T cells, S. Liénart, R. Merceron, C. Vanderaa, F. Lambert, D. Colau, J. Stockis, B. van der Woning, H. De Haard, M. Saunders, P.G. Coulie, S.N. Savvides, and S. Lucas, Science, 2018.

CXCR4-targeting nanobodies differentially inhibit CXCR4 function and HIV entry, A. Van Hout, A. Klarenbeek, V. Bobkov, J. Doijen, M. Arimont, C. Zhao, R. Heukers, R. Rimkunas, C. de Graaf, T. Verrips, B. van der Woning, H. de Haard, J.B. Rucker, K. Vermeire, T. Handel, T. Van Loya, M.J. Smit, D. Schols, Biochemical Pharmacology, 2018.

Nanobody-Fc constructs targeting chemokine receptor CXCR4 potently inhibit signaling and CXCR4-mediated HIV-entry and induce antibody effector functions, V. Bobkov, A.M. Zarca, A. Van Hout, M. Arimont, J. Doijen, M. Bialkowska, E. Toffoli, A. Klarenbeek, B. van der Woning, H.J. van der Vliet, T. Van Loy, H. de Haard, D. Schols, R. Heukers, M.J. Smit, Biochemical Pharmacology, 2018.

Cryo-EM structures of human STEAP4 reveal mechanism of iron(III) reduction, W. Oosterheert, L.S. van Bezouwen, R.N.P. Rodenburg, J. Granneman, F. Förster, A. Mattevi, and P. Gros, Nature communications, 2018.

Structures of Teneurin adhesion receptors reveal an ancient fold for cell-cell interaction, V.A. Jackson, D.H. Meijer, M. Carrasquero, L.S. van Bezouwen, E.D. Lowe, C. Kleanthous, B.J.C. Janssen, and E. Seiradake, Nature communications, 2018.

Targeting the MET oncogene by concomitant inhibition of receptor and ligand via an antibody‐“decoy” strategy, C. Basilico, C. Modica, F. Maione, E. Vigna and P.M. Comoglio, International Journal of Cancer, 2018.

A bispecific antibody strategy to target multiple type 2 cytokines in asthma, M. Godar, K. Deswarte, K. Vergote, M. Saunders, H. de Haard, H. Hammad Christophe Blanchetot, and B.N. Lambrecht, The journal of allergy and clinical Immkunology, 2018.

An Approach to Incorporate Multi-Enzyme Digestion intoC-TAILS for C-Terminomics Studies, Y. Zhang, Q. Li, J. Huang, Z. Wu, J. Huang, L. Huang, Y. Li, J. Ye, and X. Zhang, Proteomics, 2018.

Functional Characterization of Alternative and Classical Pathway C3/C5 Convertase Activity and Inhibition Using Purified Models, S.A. Zwarthoff, E.T.M. Berends, S. Mol, M. Ruyken M, P.C. Aerts, M. Józsi, C.J.C. de Haas, S.H.M. Rooijakkers, R.D. Gorham Jr, Frontiers in Immunology, 2018.

Structural insights into SorCS2–Nerve Growth Factor complex formation, N. Leloup, L.M.P. Chataigner, and B.J.C. Janssen, Nature Communications, 2018.

Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3, L. Scietti, A. Chiapparino, F. De Giorgi, M. Fumagalli, L. Khoriauli, S. Nergadze, S. Basu, V. Olieric, L. Cucca, B. Banushi, A. Profumo, E. Giulotto, P. Gissen, and F. Forneris, Nature Communications 2018.

Structures of C1-IgG1 provide insights into how danger pattern recognition activates complement, D. Ugurlar, S.C. Howes, B. de Kreuk, R.I. Koning, R.N. de Jong, F.J. Beurskens, J. Schuurman, A.J. Koster, T.H. Sharp, P.W.H.I. Parren, and P. Gros, Science, 2018.

A rationally designed NRP1-independent superagonist SEMA3A mutant is an effective anticancer agent, N. Gioelli, F. Maione, C. Camillo, M. Ghitti, D. Valdembri, N. Morello, M. Darche, L. Zentilin, G. Cagnoni, Y. Qiu, M. Giacca, M. Giustetto, M. Paques, I. Cascone, G. Musco, L. Tamagnone, E. Giraudo, and G. Serini, Science Translational Medicine 2018.

Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus, M. van Eijk, M.J. Rynkiewicz, K. Khatri, N. Leymarie, J. Zaia, M.R. White, K.L. Hartshorn, T.R. Cafarella, I. Van Die, M. Hessing, B.A. Seaton and H.P. Haagsman, Journal of Biological Chemistry, 2018.


Nogo Receptor crystal structures with a native disulfide pattern suggest a novel mode of self‐interaction, M.F. Pronker, R.P. Tas, H.C. Vlieg, and B.J.C. Janssen, Acts Crystallographica Section D, 2017.

A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels, S.A. Khetarpal,X. Zeng, J.S Millar, C. Vitali,  A.V.H. Somasundara, P. Zanoni, J.A. Landro, N. Barucci, W.J. Zavadoski, Z. Sun, H. de Haard, I.V. Toth, G.M Peloso, P. Natarajan, M. Cuchel, S. Lund-Katz, M.C. Phillips, A.R. Tall, S. Kathiresan, P. DaSilva-Jardine, N.A. Yates and D.J. Rader, Nature Medicine, 2017.

Stochastic palmitoylation of accessible cysteines in membrane proteins revealed by native mass spectrometry, R.N.P. Rodenburg, J. Snijder, M. van de Waterbeemd, A. Schouten, J. Granneman, A.J.R. Heck, and P. Gros, Nature Communications, 2017.

Recombinant Expression of the Full-length Ectodomain of LDL Receptor-related Protein 1 (LRP1) Unravels pH-dependent Conformational Changes and the Stoichiometry of Binding with Receptor-associated Protein (RAP), C. De Nardis, P. Loumlssl, M. van den Biggelaar, P.K. Madoori, N. Leloup, K. Mertens, A.J. Heck, and P. Gros, Journal of Biological Chemistry, 2017.

Interaction between Multimeric von Willebrand Factor and Complement: A Fresh Look to the Pathophysiology of Microvascular Thrombosis, S. Bettoni, M. Galbusera, S. Gastoldi, R. Donadelli, C. Tentori, G. Sparta, E. Bresin, C. Mele, M. Alberti, A. Tortajada, H. Yebenes, G. Remuzzi, and M. Noris, The Journal of Immunology, 2017.

Regulator-dependent mechanisms of C3b processing by factor I allow differentiation of immune responses, X. Xue , J. Wu, D. Ricklin, F. Forneris , P. Di Crescenzio , C.Q. Schmidt, J. Granneman, T.H. Sharp, J.D. Lambris, and P. Gros, Nature Structural and Molecular Biology, 2017.

Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release, N. Leloup, P. Lössl, D.H. Meijer, M. Brennich, A.J.R. Heck, D.M.E. Thies-Weesie, and B.J.C. Janssen, Nature Communications, 2017.

Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling, C.Y. Janda,  L.T. Dang,C. You, J. Chang, W. de Lau, Z.A. Zhong,K.S. Yan, O. Marecic, D. Siepe, X. Li, J.D. Moody, B.O. Williams, H. Clevers, J. Piehler, D. Baker, C.J. Kuo, and K.C. Garcia, Nature, 2017.


Cross-species validation of cell cycle arrest markers for acute kidney injury in the rat during sepsis, Z. Peng, F. Zhou and J.A. Kellum, Intensice Care Mediccine Experimental, 2016.

Dual Constant Domain-Fab: A novel strategy to improve half-life and potency of a Met therapeutic antibody, S. Cignettoa, C. Modica, C. Chiriaco, L. Fontani,P. Milla, P. Michieli, P.M. Comoglio, E. Vigna, Molecular Oncology, 2016.

IL4-10 Fusion Protein Is a Novel Drug to Treat Persistent Inflammatory Pain, N. Eijkelkamp, C. Steen-Louws, S.A.Y. Hartgring, H.L.D.M. Willemen, J. Prado, F.P.J.G. Lafeber, C.J. Heijnen, C.E. Hack, J.A.G. van Roon, and A. Kavelaars, The Journal of Neuroscience, 2016.

Molecular Basis of Assembly and Activation of Complement Component C1 in Complex with Immunoglobulin G1 and Antigen, G. Wang, R.N. de Jong, E.T. van den Bremer, F.J. Beurskens, A.F. Labrijn, D. Ugurlar, P. Gros, J. Schuurman, P.W. Parren, A.J. Heck, Molecular Cell, 2016.

Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis, B. Banushi, F. Forneris, A. Straatman-Iwanowska, A. Strange, A. Lyne, C. Rogerson, J.J. Burden, W.E. Heywood, J. Hanley, I. Doykov, K.R. Straatman, H. Smith, D. Bem, J. Kriston-Vizi, G. Ariceta, M. Risteli, C. Wang, R.E. Ardill, M. Zaniew, J. Latka-Grot, S.N. Waddington, S.J. Howe, F. Ferraro, A. Gjinovci, S. Lawrence, M. Marsh, M. Girolami, L. Bozec, K. Mills, and P. Gissenb, Nature Communications, 2016.

A live RSV vaccine with engineered thermostability is immunogenic in cotton rats despite high attenuation, C.C. Stobart, C.A. Rostad, Z. Ke, R.S. Dillard, C.M. Hampton, J.D. Strauss, H. Yi, A.L. Hotard, J. Meng, R.J. Pickles, K. Sakamoto, S. Lee, M.G. Currier, S.M. Moin, B.S. Graham, M.S. Boukhvalova, B.E. Gilbert, J.C.G. Blanco, P.A. Piedra, E.R. Wright, and M.L. Moore, Nature Communications, 2016.

Macrophage Stimulating Protein Enhances Hepatic Inflammation in a NASH Model, J. Li1, D. Chanda, P.J. van Gorp, M.L.J. Jeurissen, T. Houben, S.M.A. Walenbergh, J. Debets, Y. Oligschlaeger, M.J.J. Gijbels, D. Neumann, and R. Shiri-Sverdlov, PLoS ONE, 2016.

Combining somatic mutations present in different in vivo affinity-matured antibodies isolated from immunized Lama glama yields ultra-potent antibody therapeutics, A. Klarenbeek1, C. Blanchetot, G. Schragel, A.S. Sadi, N. Ongenae, W. Hemrika, J. Wijdenes, S. Spinelli, A. Desmyter, C. Cambillau, A. Hultberg, A. Kretz-Rommel, T. Dreier, H.J.W. De Haard, and R.C. Roovers, Protein Engineering, Design and Selection, 2016.

Structural Mimicry of Receptor Interaction by Antagonistic Interleukin-6 (IL-6) Antibodies, C. Blanchetot, N. De Jonge, A. Desmyter, N. Ongenae, E. Hofman, A. Klarenbeek, A. Sadi, A. Hultberg, A. Kretz-Rommel, S. Spinelli, R. Loris, C. Cambillau, and H. de Haard, JBC, 2016.

Regulators of complement activity mediate inhibitory mechanisms through a common C3b‐binding mode, F. Forneris, J. Wu, X. Xue, D. Ricklin, Z. Lin, G. Sfyroera, A. Tzekou, E. Volokhina, J.C.M. Granneman, R. Hauhart, P. Bertram, M.K. Liszewski, J.P. Atkinson, J.D. Lambris, P. Gros, The EMBO Journal, 2016.

DNA immunization combined with scFv phage display identifies antagonistic GCGR specific antibodies and reveals new epitopes on the small extracellular loops, B. van der Woning, G. de Beeck, C. Blancetot, v. Bobkov, A. Klarenbeek, M. Saunders, M. Waelbroeck, T. Laeremans, J. Steyaert, A. Hultberg and H. de Haard, Mab's, vol. 8, no. 6, 1126–1135, 2016.

Structural basis of myelin-associated glycoprotein adhesion and signalling, M.F. Pronker, S. Lemstra, J. Snijder, A.J.R. Heck, D.M.E. Thies-Weesie, R.J. Pasterkamp, B.J.C. Janssen, Nature Communications, 2016; 7: 13584.


Depleting MET-Expressing Tumor Cells by ADCCProvides a Therapeutic Advantage over Inhibiting HGF/MET Signaling, A. Hultberg, V. Morello, L. Huyghe, N. De Jonge,C. Blanchetot, V. Hanssens, G. De Boeck, K. Silence, E. Festjens, R. Heukers, B. Roux, F. Lamballe, C. Ginestier, E. Charafe-Jauffret, F. Maina, P. Brouckaert, M. Saunders, A. Thibault, T. Dreier, H. de Haard, and P. Michiel, Cancer Research, 2015.

Cryoelectron Tomography of the NAIP5/NLRC4 Inflammasome: Implications for NLR Activation, C.A. Diebolder, E.F. Halff, A.J. Koster, E.G. Huizinga, and R.I. Koning, Structure, 2015.

Cross-species validation of cell cycle arrest markers for acute kidney injury in the rat during sepsis, Z.Y. Peng, F. Zhou, and J.A. Kellum, Intensive Care Medicine Experimental, 2015.

Structural basis for inhibition of TLR2 by staphylococcal superantigen-like protein 3 (SSL3), K.J. Koymans, L.J. Feitsma, T.H. Brondijk, P.C. Aerts, E. Lukkien, P. Lössl, K.P. van Kessel, C.J. de Haas1, J.A. van Strijp, and E.G. Huizinga, PNAS, 2015.

Molecular insights into the surface-specific arrangement of complement C5 convertase enzymes, E.T. Berends, R.D. Gorham Jr, M. Ruyken, J.A. Soppe, H. Orhan, P.C. Aerts, C.J. de Haas, P. Gros, and S.H. Rooijakkers, BMC Biology, 2015.

Olfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure, M.F. Pronker, T.G.A.A. Bos, T.H. Sharp, D.M.E. Thies-Weesie, and B.J.C. Janssen, J. Biol. Chem. 2015 Jun 12; 290(24): 15092–15101.


Multimeric Growth Hormone Receptor Complexes Serve as Signaling Platforms, M. Sedek, L.M. van der Velden, and G.J. Strous, JBC, 2014.

Plasmin Cleavage of von Willebrand Factor as an Emergency Bypass for ADAMTS13 Deficiency in Thrombotic Microangiopathy, C. Tersteeg, S. de Maat, S.F. De Meyer, M.W.J. Smeets, A.D. Barendrecht, M. Roest, G. Pasterkamp, R. Fijnheer, K. Vanhoorelbeke, Ph.G. de Groot, and C. Maas, Circulation, 2014.

Fibronectin is a serum biomarker for Duchennemuscular dystrophy, F.C Martin, M. Hiller, P. Spitali, S. Oonk, H. Dalebout, M. Palmblad, A. Chaouch, M. Guglieri, V. Straub, H. Lochmuller, E.H.Niks, J.J.G.M. Verschuuren, A. Aartsma-Rus, A.M. Deelder, Y.E.M.van der Burgt, and and P.A.C.’tHoen, Proteomics Clinical Applications, 2014.

Four individually druggable MET hotspots mediate HGF-driven tumor progression, C. Basilico, A. Hultberg, C. Blanchetot, N. de Jonge, E. Festjens, V. Hanssens, S. Osepa, G. De Boeck, A. Mira, M. Cazzanti, V. Morello, T. Dreier, M. Saunders, H. de Haard and P. Michieli, J Clin Invest, 2014.

Novel Sequence Variation Affects GPIbα in Post-diarrheal Hemolytic Uremic Syndrome, E. Volokhina, A. Jakobi, R. Urbanus, E.G. Huizinga, H. Sluiter, C. Middel, D. Westra, W. van Heerde, N. van de Kar and L. van den Heuvel, J Nephrol Therapeutic, 2014.

Ensemble refinement shows conformational flexibility in crystal structures of human complement factor D, F. Forneris, B. Tom Burnley, and Piet Gros, Acta Crystallogr. D Biol. Crystallogr., 2014.

Adenovirus Composition, Proteolysis, and Disassembly Studied by In-depth Qualitative and Quantitative Proteomics, M. Benevento, S. Di Palma, J. Snijder, C.L. Moyer, V.S. Reddy, G.R. Nemerow, and A.J.R. Heck, JBC, 2014.


Molecular imaging with a fluorescent antibody targeting carbonic anhydrase IX can successfully detect hypoxic ductal carcinoma in situ of the breast, A.S.A. van Brussel, A. Adams, J.F. Vermeulen, S. Oliveira, E. van der Wall, W.P.Th.M. Mali, P.J. van Diest, and P.M.P. van Bergen en Henegouwen, Breast Cancer Research and Treatment, 2013.

Plasma reference value of heart-type fatty acid-binding protein, the earliest available plasma biomarker of acute myocardial infarction, J.F.C Glatz, and R. Mohren, Health, 5 (8), 1206 - 1209.

TLR Accessory Molecule RP105 (CD180) Is Involved in Post-Interventional Vascular Remodeling and Soluble RP105 Modulates Neointima Formation, J.C. Karper, M.M. Ewing, M.R. de Vries, S.C.A. de Jager, E.A.B. Peters, H.C. de Boer, A.J. van Zonneveld, J. Kuiper, E.G. Huizinga, T.H.C. Brondijk, J. W. Jukema, and P.H.A. Quax , PLOS one, 2013.

Near-Infrared Fluorescence Molecular Imaging of Ductal Carcinoma In Situ with CD44v6-Specific Antibodies in Mice: A Preclinical Study, J.F. Vermeulen, A.S.A. van Brussel, A. Adams, W.P.Th.M. Mali, E. van der Wall, P.J. van Diest, and P.W.B. Derksen, Mol Imaging Biol., 2013, 15(3), 290 - 298.

Proteome-derived Peptide Libraries to Study the Substrate Specificity Profiles of Carboxypeptidases, S. Tanco, J. Lorenzo, J. Garcia-Pardo, S. Degroeve, L. Martens, F.X. Aviles, K. Gevaert, and P. van Damme, Molecular and Cellular Proteomics, 2013, 12, 2096 - 2110.

Deep proteome profiling of Trichoplax adhaerens reveals remarkable features at the origin of metazoan multicellularity, J.H. Ringrose, H.W. van den Toorn, M. Eitel, H. Post, P. Neerincx, B. Schierwater, A.F. Altelaar, and A. J. Heck , Nature Communications, 2013, 4, Article 1408.

Towards Establishing a Rhinoceros-Specific Interferon-Gamma (IFN-γ) Assay for Diagnosis of Tuberculosis, D. Morar, J. Schreuder, M. Mény, P.J.S. van Kooten, E. Tijhaar, A. L. Michel, V.P.M.G. Rutten, Transboundary and Emerging Diseases, (60), Suppl 1: 60 - 60.

Structures of Wnt-Antagonist ZNRF3 and Its Complex with R-Spondin 1 and Implications for Signaling, W.C. Peng, W. de Lau, P.K. Madoori, F. Forneris, J.C.M. Granneman, H. Clevers, and P. Gros, Plos one, 8(12), e83110.

The elephant interferon gamma assay: a contribution to diagnosis of tuberculosis in elephants, T. Angkawanish, D. Morar, P. van Kooten, I. Bontekoning, J. Schreuder, M. Maas, W. Wajjwalku, A. Sirimalaisuwan, A. Michel, E. Tijhaar, and V. Rutten, Transboundary and Emerging Diseases, (60), Suppl 1: 53 - 59.

Structure of Stem Cell Growth Factor R-spondin 1 in Complex with the Ectodomain of Its Receptor LGR5, W.C. Peng, W. de Lau, F. Forneris, J.C.M. Granneman, M. Huch, H. Clevers, and P. Gros, Cell Reports, 3 (6), 1885 - 1892.


Antibody engineering reveals the important role of J segments in the production efficiency of llama single-domain antibodies in Saccharomyces cerevisiae, A. Gorlani, D. Lutje Hulsik, H. Adams, G. Vriend, P. Hermans, and T. Verrips, PEDS, 2012.

Novel C3 mutation p.Lys65Gln in aHUS affects complement factor H binding, E. Volokhina, D. Westra, X. Xue, P. Gros, N. van de Kar, and L. van den Heuvel, Pediatric Nephrology, 27, 1519 - 1524.

Fusion of the Fc part of human IgG1 to CD14 enhances its binding to Gram-negative bacteria and mediates phagocytosis by Fc receptors of neutrophils, A. Vida, B. Bardoel, F. Milder, L. Majoros, A. Sümegi, A. Bácsi, G. Vereb, K.P.M. van Kessel, J.A.G. van Strijp, and P. Antal-Szalmás, Immunology letters, 146, 31 - 39.

Near-Infrared Fluorescence Molecular Imaging of Ductal Carcinoma In Situ with CD44v6-Specific Antibodies in Mice: A Preclinical Study, J.F. Vermeulen, A.S.A. van Brussel, A. Adams, W.P.Th.M. Mali, E. van der Wall, P.J. van Diest, and P.W.B. Derksen, Molecular Imaging and Biology, 2012.

Formation and Structure of a NAIP5-NLRC4 Inflammasome Induced by Direct Interactions with Conserved N- and C-terminal Regions of Flagellin, E.F. Halff, C.A. Diebolder, M. Versteeg, A. Schouten, T.H.C. Brondijk and E.G. Huizinga, JBC, 287, 38460 - 38472.

Development of a lion-specific interferon-gamma assay, M. Maas, P.J.S. van Kooten, J. Schreuder, D. Morar, E. Tijhaar, A.L. Michel, and V.P.M.G. Rutten, Veterinary Immunology and Immunopathology, 149, (3–4), 292–297.


Enhanced secretion of leukocyte‐associated immunoglobulin‐like receptor 2 (LAIR‐2) and soluble LAIR‐1 in rheumatoid arthritis: LAIR‐2 is a more efficient antagonist of the LAIR‐1–collagen inhibitory interaction than is soluble LAIR‐1, M.J.M. Olde Nordkamp, J.A.G. van Roon, M. Douwes, T. de Ruiter, R.T. Urbanus, and L. Meyaard, Rheumatoid Arthritis, 2011.

Assessment of the Antiviral Properties of Recombinant Porcine SP-D against Various Influenza A Viruses In Vitro, M.L.B. Hillaire, M. van Eijk, S.E. van Trierum, D. van Riel, X. Saelens, R.A. Romijn, W. Hemrika, R.A.M. Fouchier, T. Kuiken, A.D.M.E. Osterhaus, H.P. Haagsman, G.F. Rimmelzwaan, PloS ONE, 6 (9), e25005.

Introduction of N-linked glycans in the lectin domain of surfactant protein D (SP-D): impact on interactions with influenza A viruses, M. van Eijk, L. Bruinsma, K.L. Hartshorn, M.R. White, M.J. Rynkiewicz , B.A. Seaton, W. Hemrika, R.A. Romijn, B.W. van Balkom, and H.P. Haagsman, JBC, 286, 20137 - 20151.

Exploring New Proteome Space: Combining Lys-N Proteolytic Digestion and Strong Cation Exchange (SCX) Separation in Peptide-Centric MS-Driven Proteomics, N. Taouatas, S. Mohammed and A.J.R. Heck, Methods in Molecular Biology, 753, 157-167.


Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex, B.J.C. Janssen, L. Gomes, R.I. Koning, D.I. Svergun, A.J. Koster, D.C. Fritzinger, C. Vogel, and P. Gros, EMBO, 28(16), 2469–2478.

Structures of C3b in Complex with Factors B and D Give Insight into Complement Convertase Formation, F. Forneris, D. Ricklin, J. Wu, A. Tzekou, R.S. Wallace, J.D. Lambris, and P. Gros, Science, 330 (6012), 1816–1820.

A Novel Disulfide Pattern in Laminin-Type Epidermal Growth Factor-like (LE) Modules of Laminin β1 and γ1 Chains, S. Kalkhof, K. Witte, C.H. Ihling, M.Q. Muller, M.V. Keller, S. Haehn, N. Smyth, M. Paulsson, and A. Sinz, Biochemistry, 49, 8359–8366.

Molecular Cloning and Functional Characterization of a Zebrafish Nuclear Progesterone Receptor, S.X. Chen, J. Bogerd, Á. García-López, H. de Jonge, P.P. de Waal, W.S. Hong, and R.W. Schulz, Biology of Reproduction, 82 (1), 171-181.

Clathrin is essential for meiotic spindle function in oocytes, J.J. Holzenspies, B.A.J. Roelen, B. Colenbrander, R.A. Romijn, w. Hemrika, W. Stoorvogel and T. van Haeften, Reproduction, 140, 223-233.

The influenza A virus hemagglutinin glycosylation state affects receptor-binding specificity, R.P. de Vries, E. de Vries, B.J. Bosch, R.J. de Groot, P.J.M. Rottier, and C.A.M. de Haan, Virology, 403, 17-25.

Studies in zebrafish reveal unusual cellular expression patterns of gonadotropin receptor messenger ribonucleic acids in the testis and unexpected functional differentiation of the gonadotropins, A. Garcia-Lopez, H. de Jonge, R.H. Nobrega, P.P. de Waal, W. van Dijk, W. Hemrika, G.L. Taranger, J. Bogerd, and R.W. Schulz, Endocrinology, 151 (5), 2349-2360.

Purification of parvalbumin from Carp: A protocol that avoids heat-treatment, S.J. Koppelman, R.A. Romijn, H.H.J. de Jongh, J.A. Nordlee, S. Piersma, M. Hessing and S.L. Taylor, Journal of food Science, 75 (3), T49-T56.

Molecular Cloning and Functional Characterization of a Zebrafish Nuclear Progesterone Receptor, S.X. Chen, J. Bogerd, Á. García-López, H. de Jonge, P.P. de Waal, W.S. Hong, and R.W. Schulz, Biology of Reproduction, 82 (1), 171-181.


Structural and functional implications of the alternative complement pathway C3 convertase stabilized by a staphylococcal inhibitor, S.H.M. Rooijakkers, J. Wu, M. Ruyken, R. van Domselaar, K.L. Planken, A. Tzekou, D. Ricklin, J.D. Lambris, B.J.C. Janssen, J.A.G. van Strijp and P. Gros, Nature Immunology, 10 (7), 721-729.

Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis, P.P. de Waal, M.C. Leal, Á. García-López, S. Liarte, H. de Jonge, N. Hinfray, F. Brion, R.W. Schulz, and J. Bogerd, Journal of Endocrinology, 202, 287-297.

Staphylococcal SSL5 inhibits leukocyte activation by chemokines and anaphylatoxins, J. Bestebroer, K.P.M. van Kessel, H. Azouagh, A.M. Walenkamp, I.G.J. Boer, R.A. Romijn, J.A.G. van Strijp and C.J.C. de Haas, Blood, 113 (2), 328-337.

Staphylococcal Superantigen-like10 Inhibits CXCL12-Induced Human Tumor Cell Migration, A.M.E. Walenkamp, I.G.J. Boer, J. Bestebroer, D. Rozeveld, H. Timmer-Bosscha, W. Hemrika, J.A.G. van Strijp and C.J.C. de Haas, Neoplasia, 11 (4), 333-344.

Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex, B.J.C. Janssen, L. Gomes, R.I. Koning, D.I. Svergun, A.J. Koster, D.C. Fritzinger, C. Vogel and P. Gros, EMBO, 28, 2469-2478.

Structure and functional relevance of the Slit2 homodimerization domain, E. Seiradake, A.C. von Philipsborn, M. Henry, M. Fritz, H. Lortat-Jacob, M. Jamin, W. Hemrika, M. Bastmeyer, S. Cusack and A.A. McCarthy, EMBO, 10, 736-741.


cGMP-binding Prepares PKG for Substrate Binding by Disclosing the C-terminal Domain, V. Alverdi, H. Mazon, C. Versluis, W. Hemrika, G. Esposito, R. van den Heuvel, A. Scholten, A.J.R. Heck, Journal of Molecular Biology, 104 (38), 14923-14928.

Chicken lung lectin is a functional C-type lectin and inhibits haemagglutination by influenza A virus, A. Hogenkamp, N. Isohadouten, S.S.N. Reemers, R.A. Romijn, W. Hemrika, M.R. White, B. Tefsen, L. Vervelde, M. van Eijk, E.J.A. Veldhuizen, and H.P. Haagsman, Veterinary Microbiology, 130, 37-46.

Platelet activation by dimeric beta2-glycoprotein I requires signaling via both glycoprotein Ibalpha and apolipoprotein E receptor 2', R.T. Urbanus, M.T. Pennings, R.H. Derksen, and Ph.G. De Groot,  Journal of Thrombosis and Haemostasis, 6 (8), 1405 - 1412.

In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor, B. Arends, B. Spee, G. Hoffmann, G.E.G. Jansen, E. Slump, E. Auriemma, J. IJzer, W. Hemrika, R.A. Romijn, K.H.A. van der Heijden-Liefkens, P.J.A. Sondermeijer, T.S.G.A.M. van den Ingh, L.C. Penning, and J. Rothuizen, The Veternary Journal, 178, 70-77.

Crystal structures of TAFI elucidate the inactivation mechanism of activated TAFI; A novel mechanism for enzyme auto-regulation, P.F. Marx, T.H.C. Brondijk, T. Plug, R.A. Romijn, W. Hemrika , J.C.M. Meijers and E.G. Huizinga, Blood, 112 (7), 2803-2809.

Cathepsin L functionally cleaves the SARS-CoV class I fusion 1 protein upstream of rather than adjacent to the fusion peptide, B.J. Bosch, W. Bartelink and P. Rottier, Journal of Virology, 82 (17), 8887-8890.


Factor B structure provides insights into activation of the central protease of the complement system , F.J. Milder, L. Gomes, A. Schouten, B.J.C. Janssen, E.G. Huizinga, R.A. Romijn, W. Hemrika, A. Roos, M.R. Daha and P. Gros, Nature Structural & Molecular Biology, 14, 224 - 228.

Surface-Plasmon-Resonance-Based Chemical Pteomics: Efficient Specific Extraction and Semiquantitative Identification of Cyclic Nucleotide-Binding Proteins from Cellular Lysates by Using a Combination of Surface Plasmon Resonance, Sequential Elution and Liquid Chromatography-Tandem Mass Spectrometry, N.F.C. Visser, A. Scholten, R.H.H. van den Heuvel, and A.R.J. Heck, ChemBioChem, 8, 298 - 305.

The Beta-Defensin Gallinacin-6 Is Expressed in the Chicken Digestive Tract and Has Antimicrobial Activity against Food-Borne Pathogens, A. van Dijk, E.J.A. Veldhuizen, S.I.C. Kalkhove, J.L.M. Tjeerdsma-van Bokhoven, R.A. Romijn and Henk P. Haagsman, Antimicrobal agents and chemotherapy, 51-3, 912-922.

Platelets express three different splice variants of ApoER2 that are all involved in signaling, M.T.T. Pennings, R.H.W.M. Derksen, R.T. Urbanus, W.L. Tekelenburg, W. Hemrika, Ph.G. de Groot, Journal of Thrombosis and Haemostasis, 5, 1538 - 1544.

Cloning, expression, crystallization and preliminary X-ray analysis of the first two Ig domains from human Roundabout 1 (Robo1), C. Morlot, W. Hemrika, R.A. Romijn, P. Gros, S. Cusack and A.A. McCarthy, Acta Cryst. F63, 689 - 691.

Production of Slit2 LRR domains in mammalian cells for structural studies and the structure of human Slit2 domain 3, C. Morlot, W. Hemrika, R.A. Romijn, P. Gros, S. Cusacka, and A.A. McCarthy, Acta Crystallogr D. Biol Crystallography, Sep., 63(Pt 9), 961-968.

Structural insights into the Slit-Robo complex, C. Morlot, N.M. Thielens, R.B.G. Ravelli, W. Hemrika, R.A. Romijn, P. Gros, S. Cusack and A.A. McCarthy, PNAS, 104 (38), 14923-14928.


Interaction of ß2-glycoprotein I with members of the low density lipoprotein receptor family, M.T.T. Pennings, M. van Lummel, R.H.W.M. Derksen, R.T. Urbanus, R.A. Romijn, P.J. Lenting and Ph.G. de Groot, Journal of Thrombosis and Haemostasis, 4, 1680 - 1690.

Origin and evolution of the peroxisomal proteome, Toni Gabaldón, Berend Snel, Frank van Zimmeren, Wieger Hemrika, Henk Tabak and Martijn A Huynen, Biology Direct 2006, 1:8 (23 March 2006).

Analysis of the cGMP/cAMP Interactome Using a Chemical Proteomics Approach in Mammalian Heart Tissue Validates phingosine Kinase Type 1-interacting Protein as a Genuine and Highly Abundant AKAP, Arjen Scholten, Mee Kian Poh, Toon A. B. van Veen, Bas van Breukelen, Marc A. Vos, and Albert J. R. Heck, Journal of Proteome Research 2006, 5, 1435-1447.

Characterization of bitiscetin-2, a second form of bitiscetin from the venom of Bitis arietans: comparison of its binding site with the collagen-binding site on the von Willebrand factor A3-domain, B. Obert, R.A. Romijn , A. Houllier, E.G. Huizinga and J.P. Girma, Journal of Thrombosis and Haemostasis, 4, 1596-1601.

VWF A1 domain can adequately substitute for A3 domain in recruitment of flowing platelets to collagen, Arnaud Bonnefoy, Roland A. Romijn, Petra A.H. Vandervoort, Isabel Van Rompaey, Jos Vermylen and Marc F. Hoylaerts, Journal of Thrombosis and Haemostasis, 4, 2151 - 2161.

Structure of Complement Component C2a: Implications for Convertase Formation and Substrate Binding, Fin J. Milder, Hans C.A. Raaijmakers, Mitja D.A.A. Vandeputte, Arie Schouten, Eric G. Huizinga, Roland A. Romijn, Wieger Hemrika, Anja Roos, Mohamed R. Daha and Piet Gros, Structure, 14, 1587-1597.


The binding site in beta2-glycoprotein I for apoER2' on platelets is located in domain V, M. van Lummel, M.T.T. Pennings, R.H.W.M. Derksen, R.T. Urbanus, B.C.H. Lutters, N. Kaldenhoven, and P.G. de Groot, JBC, 280(44), 36729-3673.


Mapping the collagen-binding site in the von Willebrand Factor A3-domain. R.A. Romijn, Erik Westein, Barend Bouma, Marion E. Schiphorst, Jan J. Sixma, Peter J. Lenting and Eric G. Huizinga, Journal of Biological Chemistry, 278(17), 15035-15039.


Structures of Glycoprotein Iba and its complex with von Willebrand Factor A1 domain. E.G. Huizinga, S. Tsuji, R.A. Romijn, J.J. Sixma and P. Gros, Science, 2002, 297, 1176-1179.


Identification of the Collagen-binding Site of the von Willebrand Factor A3-domain. R.A. Romijn, B. Bouma, W. Wuyster, P. Gros, J. Kroon, J.J. Sixma and E.G. Huizinga, Journal of Biological Chemistry, 2001, 276(13), 9985-9991.